How to Make Measurements on Biological Samples

When it comes to making measurements on biological samples at the nanoscale, Atomic force microscopes are used. In both ambient atmospheric conditions as well as liquid environments, AFM allows the nanoscale imaging of soft biomaterials including cells and DNA.

Have a look at the biology applications, unique to atomic force microscopes:

Double-stranded DNA Molecules

Imaging of DNA of size 5 µm x 5 µm can be done easily. Imaging of oligonucleotides can be used in a wide variety of applications, notably in structural studies, and in the study of interactions between the oligonucleotides and other molecules, such as enzymes.

Tobacco Mosaic Virus (TMV)

Imaging of Tobacco Mosaic Virus is commonly carried out in structural studies. Such high-resolution imaging requires an instrument noise floor of less than 0.1nm, a good tip approach leading to the maintenance of a sharp tip, the capability to scan with very light forces and placement of the microscope in an environment with minimal structural and acoustic vibrations. Furthermore, TMV is commonly used as an imaging standard since it has a highly conserved structure.

Parasites

Parasites of size 25 µm x 25µm can be measured in the air using AFM. These Leishmania cells have been treated with an antimicrobial peptide, leading to highly roughened cell membranes, which can be measured and quantified by AFM.

Epithelial Cells

Epitheleal cell of size 32 µm x 32 µm can be measured in liquid using Atomic Force Microscope. Measurement of high-resolution images of cells in a liquid (e.g., under physiological conditions) is another possibility unique to AFM and can give much more relevant results than electron microscopy, which requires cell fixation, leading to artifacts.

Atomic force microscopy has a particular advantage over electron microscopy, in that cells and biomaterials can be imaged in partially or totally hydrated conditions including ambient air and liquid environments.

Recommended AFM products for life sciences applications are TT-2 Atomic Force Microscope and LS-Atomic Force Microscope. To buy high-quality Atomic Force Microscope, feel free to visit https://www.afmworkshop.com/.

Buy AFMWorkshop Microscopes to Handle Your Nanotechnology Research Needs

For nanotechnology research, Atomic force microscopes are essential for the visualization and measurement of nanostructures.

AFM Workshop’s innovative microscopes offer a balance between an affordable price and the rigorous performance required by many nanotechnology researchers. Their Atomic force microscopes offer the necessary resolution for the measurement of nanostructures including nanoparticles, DNA, thin films, polymers, and much more. AFM Workshop microscopes with noise floors as low as 0.08 nm, are fully capable of handling your nanotechnology research needs.

All the products offered by AFM Workshop are robust and can be used in single-user as well as multi-user laboratories. AFMWorkshop’s microscopes share a powerful and intuitive user interface that meets the needs of casual as well as advanced AFM operators.

Atomic Force Microscopes give great images of nanostructures on both soft and hard materials. AFMWorkshop microscopes have an open architecture to facilitate the development of novel instruments. These microscopes can be used by AFMWorkshop customers to create images used in publications.

AFMWorkshop products are widely used every day in both life and physical sciences throughout the world for nanotechnology research projects. AFMWorkshop products were introduced to the market in late 2010. They already have a growing list of almost 200 publications written by successful AFMWorkshop customers. To date there are 250 AFMWorkshop microscopes installed in laboratories.

When it comes to scanning samples, almost all type of samples including nanostructures, patterned surfaces, materials, and life science samples are scanned with AFMWorkshop microscopes. AFMWorkshop’s TT-AFM is a particularly useful AFM for labs with much more expensive Atomic Force Microscopes that are already dedicated to specialized experiments.

If you are looking for top-quality atomic force microscopes then get in touch with AFMWorkshop. AFMWorkshop guarantees their AFMs will run your application, and their customer service is always available to assist.

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Applications of Atomic Force Microscopy

For visualizing nanostructures and making measurements at the nano scale, atomic force microscopes (AFM) are an essential tool. AFMs are routinely used by scientists and researchers throughout the world to visualize surface structure and to make dimensional measurements.

Atomic Force Microscopes have a wide range of applications in physical sciences, life sciences and all disciplines of engineering. Here are a few of the common applications for atomic force microscopes:

Nanotechnology

Nanotechnology is an almagum of all disciplines of science and engineering, Atomic Force Microscopes are key nanoscale measurement instruments facilitating nanotechnology developments. Atomic force microscopes are essential tools for nanotechnology research, providing the necessary resolution for the visualization and measurement of nanostructures including nanoparticles, DNA, thin films, polymers, and more.

Life Sciences

AFMs are effective tool for scanning biological samples that are not possible to scan with any other type of microscope. Biomaterials, cells, and other soft samples can be scanned by AFMs in ambient air as well as liquids. Besides measuring 3-D images an afm can measure parameters including stiffness and adhesion.

Process Development/Control

Atomic force microscope is finding acceptance for evaluating samples for process development, and to control a process. Accuracy and precision can be assured by using standard measurement protocols and qualified probe tips. AFM measurements used in process control are typically made repetitively.

Instrument Innovation

Atomic force microscopes are ideal for instrument builders and engineers. They can be used as a platform for creating new instrumentation, such as new imaging modes. AFM can also be used in combination with another analytical instrument such as a mass spectrometer, nanoindenter, and IR spectrometer.

Nanoparticles

Atomic force microscopy can be used for Nanoparticle characterization. Often with an AFM information is gained that cannot be measured with dynamic light scattering, electron microscopy, and other optical characterization methods. AFM allows 3D characterization of nanoparticles with sub-nanometer resolution.

Photonics

AFMs are ideal for the analysis of materials used in the photonics industry. Thus, it offers substantially better horizontal and vertical resolution than optical and stylus profilers.

Polymer Characterization

Atomic force microscopy is an ideal method for imaging polymers, imaging polymers blends, and polymer composites with nanometer lateral resolution. For polymer applications, the AFM gives better resolution than optical microscopy and unlike in a scanning electron microscope, an AFM does not require coating the sample before imaging.

If you want to learn more about Atomic Force Microscopes (AFMs) applications or AFMWorkshop products, contact AFMWorkshop or one of our distribution partners. For more information, please visit https://www.afmworkshop.com.